| T O P I C R E V I E W |
| vsoza |
Posted - March 02 2020 : 2:05:56 PM
Has anyone had trouble getting good DNA yield from fresh-collected Rhododendron samples? If so, have you found a good protocol that has worked for problematic samples? |
| 5 L A T E S T R E P L I E S (Newest First) |
| RhodoRyan |
Posted - May 14 2020 : 5:14:25 PM
I haven't had a lot of issues with extraction, but I know a member of our lab had some issues with Qiagen.
We most recently used this kit for Target-Capture sequencing with great results:
https://www.asone-int.com/product/invisorb-spin-plant-mini-kit-2/
If you want a cheaper route, we have some CTAB protocols that also worked well for troublesome samples. Email me and I can send them over.
-Ryan |
| JulianaMedeiros |
Posted - April 30 2020 : 09:59:07 AM
Glad you both like the Quiagen DNEasy, because I have a bunch of those purchased that Im planning to use on R. minus |
| emilygillespie |
Posted - April 29 2020 : 11:24:12 AM
I've used more Qiagen DNEasy than anything over the past decade with good results. I modified for herbarium materia or silica-dried material according to Drabkova et al (2002?)...though their work was done on Juncus or something.
quote:
Originally posted by vsoza
Has anyone had trouble getting good DNA yield from fresh-collected Rhododendron samples? If so, have you found a good protocol that has worked for problematic samples?
|
| JulianaMedeiros |
Posted - March 10 2020 : 1:06:05 PM
We've had good luck with phenol cholorform extractions, although we've only tried it on a few species. |
| vsoza |
Posted - March 02 2020 : 2:11:46 PM
We have used the Qiagen DNeasy kit but have used ethanol precipitations instead of column cleanup because DNA in vireyas was not binding to the columns.
quote:
Originally posted by vsoza
Has anyone had trouble getting good DNA yield from fresh-collected Rhododendron samples? If so, have you found a good protocol that has worked for problematic samples?
|
